期刊论文

Effects of Dendrobium officinale polysaccharides on gut microbiota of mice

中文

湖南农业大学学报(自然科学版)

1007-1032

2021

47

4

449-454

10.13331/j.cnki.jhau.2021.04.013

国家级大学生创新创业训练计划项目（201910541033）； 湖南中医药大学研究生培养质量工程项目（2019CX08）；

本校为第一机构

将10只雄性KM小鼠随机分为正常组及铁皮石斛多糖组,铁皮石斛多糖组小鼠按临床等效剂量(350 mg/kg)灌胃8周,每天1次,每次每只0.4 mL,正常组小鼠灌胃等体积无菌水,灌胃结束后取肠道内容物及黏膜进行16S rRNA基因测序并进行功能预测,探讨铁皮石斛多糖对正常小鼠肠道菌群的影响.结果表明:铁皮石斛多糖提高了肠腔菌群的α多样性,并显著提高了肠黏膜菌群的α多样性,但β多样性的变化无统计学意义;铁皮石斛多糖使肠腔中Diaphorobacter,肠黏膜中Muribaculaceae、Staphylococcus的相对丰度显著增加,并增加了Akkermansia、Phascolarctobacterium、Turicibacter、Faecalibacteriu、Parabacteroides、Parasu...

10 male KM mice were divided into normal group and Dendrobium officinale polysaccharides(DOP) treated group. Mice in the treated group were gavaged with DOP at the clinical equivalent dose(350 mg/kg) for 8 weeks, once a day, 0.4 mL each rat at a time, and the control group was gavaged with sterile water. To investigate the effect of DOP on gut microbiota of mice, the intestinal contents and mucosa were collected for 16S rRNA gene sequencing and function prediction. The results showed that DOP increased α diversity of lumen-associated microbiot...