目的:建立测定小鼠肝脏中16种胆汁酸浓度的LC-MS/MS法,并将测定结果应用于比较Nrf2野生型和基因敲除型小鼠胆汁酸代谢谱。方法:小鼠肝脏样品加入70%甲醇匀浆,用甲醇沉淀匀浆液中的蛋白并高速离心,取上清液入进样瓶,用XtimateTMC18柱分离,以甲醇-醋酸铵及甲酸水溶液为流动相进行梯度洗脱,流速0.25 m L·min-1,进样5μL分析。结果:16种胆汁酸在0.013~8.000μmo
摘要(英文)：
Objective: To determine the concentration of 16 bile acids in mice liver by LC-MS/MS.Methods: After added 70 % methanol into mice liver,protein precipitation with methanol was used for sample preparation.Liquid chromatographic separation was performed on a XtimateTM C18 column with gradient eluent consisting of methanol-ammonium acetate and formic acid buffer,and the flow rate was 0.25 mL·min-1 and injection volume was 5 μL.Results: The linear calibration curves were obtained in the concentration range of 0.013~8.000 μmol·L-1.The lower ...
