期刊论文

Effects of CurTn on proliferation of VSMC

中文

中国药理学通报

1001-1978

2016

32

11

1526-1530

10.3969/j.issn.1001-1978.2016.11.010

国家自然科学基金资助项目（No81673722 816700291 81670268） 湖南省自然科学基金资助项目（No14JJ1024 2015JJ2117） 湖南省教育厅重点项目（No16A156）.致谢：本实验主要在湖南中医药大学干细胞调控与应用实验室及南华大学药物药理研究所完成,在此对实验室各位老师及同学的帮助表示衷心的感谢!

本校为第一机构

目的探讨姜黄素烟酸酯( curcumin trinicotinate, CurTn)对血管平滑肌细胞( vascular smooth muscle cell, VSMC)增殖的影响及其可能机制。方法体外培养VSMC,体积分数为0.1的 FBS 刺激 VSMC 生长。 MTT 法观察CurTn对VSMC 活力的影响；流式细胞术分析细胞周期；Western blot 检测 PCNA、p-ERK1/2、CyclinD1蛋白的表达。结果 CurTn抑制VSMC增殖并呈现一定量效、时效关系,并且使G1期的细胞比例增加,S期细胞比例下降,PCNA蛋白表达下调。同时发现 CurTn 能明显抑制 p-ERK1/2、Cy-clinD1蛋白表达。结论 CurTn能明显抑制VSMC增殖,其作用机制可能与p-ERK1/2、CyclinD1蛋白表达下调有关。

Aim To explore the anti-proliferation effects of curcumin trinicotinate (CurTn) on vascular smooth muscle cell (VSMC) and its mechanism. Methods The cells were cultured in DM EM supplemented with 10% fetal bovine serum. MTT assay was used to examine cell proliferation. FCM was used to observe cell cycle. The expressions of PCNA, Cy-clinDl and p-ERKl/2 were analyzed using Western blot. Results CurTn could inhibit the proliferation of VSMC and showed a certain amount-time relationship. What's more, CurTn could increase the G, phase proportion of cell, decrease the S phase proportion and the expr...