期刊论文

中文

中国药理学通报

1001-1978

2017

33

10

1467-1472

10.3969/j.issn.1001-1978.2017.10.027

国家自然科学基金资助项目（No 81303078） 湖南省科技厅重点项目（No 2014SK2007） 湖南省教育厅优秀博士学位论文获得者科研项目（No YB2016B030） 湖南省研究生科研创新项目（No CX2015B331）

本校为第一机构

目的研究益气活血中药脑泰方通过Keap1-Nrf2/ARE信号通路对脑缺血/再灌注后大鼠海马区血红素加氧酶-1(heme oxygenase-1,HO-1)和膜铁转运辅助蛋白(hephaestin,Heph)表达的影响。方法将80只♂大鼠随机分为假手术组、模型组、脑泰方低剂量组(4.5 g·kg~(-1))、中剂量组(9 g·kg~(-1))和高剂量组(18 g·kg~(-1))。各组大鼠术前连续灌胃给药3 d,每日1次,再行大脑中动脉线栓法脑缺血/再灌注模型制备术,术后连续灌胃给药2 d。术后72 h采用Zea Longa神经功能学评分标准记录大鼠行为活动,TTC染色法测定脑梗死体积,real-time PCR检测大鼠海马区Nrf2、HO-1、Heph的mRNA表达,Western blot检测Nrf2、HO-1、H...

Aim To investigate the effects of Naotai formula extract ( NTE) on the expression of heme oxygenase-1 (HO-1) and hephaestin( Heph) in hippocampus of rats after cerebral ischemia/reperfusion by Keapl-Nrf2/ARE signaling pathway. Methods Eighty rats were randomly divided into five groups as follows: sham operation group ( Sham ), cerebral ischemia/reperfusion group(I/R), low dose group of NTE(4.5 g kg"' ), middle dose group of NTE (9 g kg") and high dose group of NTE( 18 g kg"' ). Rats were prelreated by intragastric administration for three consecutive days, and then subjected to middle cerebral...