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MAPK/ERK信号通路在益气解毒方水提物诱导鼻咽癌细胞凋亡中的作用

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成果类型:
期刊论文
论文标题(英文):
Role of MAPK/ERK signaling pathway in apoptosis of nasopharyngeal carcinoma cells by Yiqi Jiedu formula
作者:
胡晶;刘洁;徐冰雁;戴娜;胡梅;...
通讯作者:
He, Y.
作者机构:
Post-Graduate School, Hunan University of Chinese Medicine, Changsha, 410208, China
Hunan Provincial Key Lab for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China
[胡梅] College of Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China
Hunan Provincial Research Engineering Technology Center for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine and Visual Function Protection, Hunan University of Chinese Medicine, Changsha, 410208, China
College of traditional Chinese Medicine, Changsha Medical University, Changsha, 410219, China
通讯机构:
[He, Y.] H
Hunan Provincial Key Lab for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China
语种:
中文
关键词:
益气解毒方;鼻咽癌;细胞凋亡;MAPK/ERK信号通路
关键词(英文):
Survivin;XIAP
期刊:
中国药理学通报
ISSN:
1001-1978
年:
2019
卷:
35
期:
11
页码:
1613-1621
基金类别:
No81573721:国家自然科学基金 81874408:国家自然科学基金 No2016JJ6117:湖南省自然科学基金 2017JJ3246:湖南省自然科学基金 No2017CX33:研究生创新项目
机构署名:
本校为第一且通讯机构
院系归属:
医学院
研究生院
摘要:
目的 研究益气解毒方水提物对鼻咽癌细胞凋亡的影响,并从MAPK/ERK信号通路探讨其诱导凋亡的作用机制。方法 CCK-8法检测益气解毒方水提物对CNE1、CNE2细胞增殖的影响;Hoechst 33342染色法、JC-10染色法、荧光双染流式细胞仪检测其对CNE1、CNE2细胞凋亡的影响;Western blot法检测其对CNE1、CNE2细胞蛋白表达的影响。结果 益气解毒方水提物能抑制CNE1、CNE2细胞增殖( P
摘要(英文):
;Aim To study the effect of Yiqi Jiedu formula extract on the apoptosis of nasopharyngeal carcinoma cells, and explore the mechanism of its induction of apoptosis from MAPK/ERK signaling pathway. Methods The effect of Yiqi Jiedu Formula extract on the proliferation of CNE1 and CNE2 cells was detected by CCK-8. The apoptosis of CNE1 and CNE2 cells was detected by Hoechst 33342 staining, JC-10 staining and fluorescence double dye flow cytometer staining. The protein expression of CNE1 and CNE2 cells was detected by Western blot. Results Yiqi Jied...

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