期刊论文

Role of MAPK/ERK signaling pathway in apoptosis of nasopharyngeal carcinoma cells by Yiqi Jiedu formula

He, Y.

中文

中国药理学通报

1001-1978

2019

35

11

1613-1621

10.3969/j.issn.1001-1978.2019.11.024

No81573721:国家自然科学基金 81874408:国家自然科学基金 No2016JJ6117:湖南省自然科学基金 2017JJ3246:湖南省自然科学基金 No2017CX33:研究生创新项目

本校为第一且通讯机构

目的研究益气解毒方水提物对鼻咽癌细胞凋亡的影响,并从MAPK/ERK信号通路探讨其诱导凋亡的作用机制。方法CCK-8法检测益气解毒方水提物对CNE1、CNE2细胞增殖的影响; Hoechst 33342染色法、JC-10染色法、荧光双染流式细胞仪检测其对CNE1、CNE2细胞凋亡的影响; Western blot法检测其对CNE1、CNE2细胞蛋白表达的影响。结果益气解毒方水提物能抑制CNE1、CNE2细胞增殖(P < 0.05)、诱导凋亡(P < 0.05);药物作用48 h后,Survivin、XIAP、Bcl-2表达下降,Bax表达上升,MAPK/ERK信号通路关键蛋白p-c-Raf、p-MEK、p-ERK表达下降(P < 0.05);在此基础上,加入激活剂ISO和益气解毒方水提物后,与单用益气解毒方水提...

;Aim To study the effect of Yiqi Jiedu formula extract on the apoptosis of nasopharyngeal carcinoma cells, and explore the mechanism of its induction of apoptosis from MAPK/ERK signaling pathway. Methods The effect of Yiqi Jiedu Formula extract on the proliferation of CNE1 and CNE2 cells was detected by CCK-8. The apoptosis of CNE1 and CNE2 cells was detected by Hoechst 33342 staining, JC-10 staining and fluorescence double dye flow cytometer staining. The protein expression of CNE1 and CNE2 cells was detected by Western blot. Results Yiqi Jied...