期刊论文

Effect of borneol combined with astragaloside IV and Panax notoginseng saponins on permeability of blood - brain barrier after cerebral ischemia - reperfusion

中文

中国药理学通报

1001-1978

2019

35

11

1516-1523

10.3969/j.issn.1001-1978.2019.11.008

国家自然科学基金资助项目(No 81573875) 湖南省科技厅科技创新平台与人才计划-中医脑病临床研究中心(No 2017SK4005) “中西医结合防治心脑血管疾病的相关基础研究” “中医药防治心脑血管疾病基础研究”湖南省自然科学创新群体基金 湖南中医药大学“十三五”一级学科基础医学建设项目(No 06) 湖南省教育厅一般项目(No 18C0407).

本校为第一机构

目的探讨冰片、黄芪甲苷(AST Ⅳ)和三七总皂苷(PNS)配伍对脑缺血/再灌注后血脑屏障(BBB)的影响。方法脑缺血/再灌注大鼠灌胃给药,观察神经功能缺损症状,测定脑组织含水量和BBB通透性、闭锁小带蛋白1(ZO-1)、 ZO-2、咬合蛋白(Occludin)及闭合蛋白5(Claudin-5)表达。结果脑缺血/再灌注后,大鼠出现神经功能缺损症状,神经功能评分和脑含水量增加,BBB破坏。各药物能不同程度减轻上述病理改变,冰片+ AST Ⅳ+ PNS效应最强,优于药物单用及AST Ⅳ+ PNS。脑缺血/再灌注后,ZO-1、ZO-2、Occludin、Claudin-5表达减少。各药物使ZO-1增加,除AST Ⅳ外,上调Occludin表达;冰片+ AST Ⅳ+ PNS还明显上调ZO-2,且上调...

Aim To probe the effect of borneol combined with astragalosides IV (AST I V) and Panax notoginseng saponins (PNS) on blood-brain barrier (BBB) after cerebral ischemia-reperfusion. Methods Rats with cerebral ischemia-reperfusion were administered by gavage, the symptom of nerve function defect was observed, the water content of brain tissues, the permeability of BBB, and the expression of zonula cccludens 1 (ZO-1), ZO-2, Occludin and Claudin-5 were detected. Results After cerebral ischemia-reperfusion, the neurological deficit symptom appeared, ...