期刊论文

Effects of Huangqi Jianzhong decoction on duodenal ulcer and TLR-2 mediated intestinal mucosal immune barrier in rats

中文

中国药理学通报

1001-1978

2019

35

8

1172-1178

10.3969/j.issn.1001-1978.2019.08.026

国家自然科学基金资助项目(No 81703920) 湖南省中医药管理局一般项目(No 201780) 湖南省大学生研究性学习和创新性实验计划项目(No 2017280) 湖南省自然科学基金项目(No 2019JJ50442).

本校为第一机构

目的 研究黄芪建中汤(Huangqi Jianzhong decoction,HQJZ)对大鼠十二指肠溃疡(duodenal ulcer,DU)的治疗效应及其对TLR-2介导的肠黏膜免疫屏障功能的影响.方法 将大鼠分为正常组、模型组、阳性药组和HQJZ组,采用复合病因法构建DU模型.观测大鼠整体状态;对DU损伤进行评分;HE染色观察十二指肠病理改变;ELISA法检测细胞因子含量;荧光定量PCR和免疫组化法分别检测mRNA及蛋白表达.结果 与正常组比较,模型大鼠十二指肠黏膜发生明显溃疡病变;血清IL-4、IL-10含量明显下降,TNF-α 含量明显升高;黏膜TLR-2、MyD88 mRNA及蛋白表达明显上调.给予HQJZ治疗后,大鼠体质量、进食量、肛温明显回升;溃疡指数明显降...

Aim To study the therapeutic effect of Huangqi Jianzhong decoction(HQJZ) on duodenal ulcer(DU) in rats and its effect on intestinal mucosal immune barrier function mediated by TLR-2.Methods SD rats were divided into normal group,model group,positive drug group and HQJZ group.DU model was established by methods of multiple factors.The body weight,food intake,and rectal temperature were measured.The ulcer index(UI) was calculated.The pathological changes of duodenal mucosa were observed by HE staining.The contents of cytokines were detected by EL...