期刊论文

Jinguishenqi decoction promotes autophagy of renal podocytes in diabetic mice

中文

中国药理学通报

1001-1978

2019

35

3

430-435

10.3969/j.issn.1001-1978.2019.03.026

No81603470:国家自然科学基金 81503442:国家自然科学基金

本校为第一机构

目的 探讨金匮肾气汤对db/db糖尿病小鼠肾脏足细胞自噬的影响及机制.方法 将6周龄db/db糖尿病小鼠随机分为生理盐水组、金匮肾气汤低、高剂量组、二甲双胍组,均灌胃给药;♂db/m小鼠作为正常对照组.于第18周龄处死动物,留24 h尿检测尿白蛋白、肌酐;取血,检测血糖、肌酐;留取肾脏组织,分别进行PAS染色观察肾小球内细胞外基质增生情况,免疫组织化学检测WT1在肾组织的表达,透射电子显微镜观察足细胞内自噬体情况,Western blot检测LC3Ⅰ/Ⅱ、p62、p-mTOR/mTOR、p-AMPK/AMPK在肾组织的表达.结果 与db/db糖尿病小鼠组比较,金匮肾气汤治疗后,24 h尿白蛋白减少、尿白蛋/肌酐下降、血肌酐下降(P

Aim: To investigate the effect of Jinguishenqi decoction on renal podocyte autophagy in db/db diabetic mice and its mechanism. Methods: Twenty 6-week old db/db diabetic mice were randomly divided into four groups, with five mice in each group: saline group, Jinguishenqi decoction low dose group, Jinguishenqi decoction high dose group, metformin positive control group, and 5 male db/m mice as normal control group. The animals were sacrificed at 18 weeks old, and urinary albumin and creatinine were detected in 24 hours' urine. Blood glucose and c...