期刊论文

Increased LDL-C uptakes of hepatocytes treated by curcumin via downregulating PCSK9 expression

中文

中国药理学通报

1001-1978

2020

36

10

1415-1421

10.3969/j.issn.1001-1978.2020.10.016

国家自然科学基金资助项目(No 81600291,31871169) 湖南省自然科学基金资助项目(No 2018JJ2348,2018JJ2346,2018JJ2300) 湖南省研究生科研创新项目资助(No CX2018B625) 湖南省教育厅优秀青年项目(No 16B272)。

本校为其他机构

目的明确姜黄素调节PCSK9促进肝细胞摄取LDL的作用。方法利用实验室现有的慢病毒感染HepG2和LO2两种细胞,分别构建过表达PCSK9(RNAi-PCSK9)和干扰PCSK9(OE-PCSK9)细胞模型,通过倒置荧光显微镜检测慢病毒转染效率;Western blot检测蛋白表达;油红O染色分析姜黄素对细胞中脂质摄取情况;酶法检测细胞内胆固醇含量。结果镜下观察细胞内可见清晰绿色荧光。Western blot结果显示干扰PCSK9组降低PCSK9表达,升高LDLR表达;过表达PCSK9组增加PCSK9表达,降低LDLR表达。油红O结果显示姜黄素/瑞舒伐他汀处理24 h后RNAi/OEPCSK9组细胞内脂质含量均增加。胆固醇含量测定姜黄素/瑞舒伐他汀处理24 h后RNAi-PCSK9组...

Aim To confirm the role of curcumin in promoting uptake LDL of hepatocytes via regulating PCSK9. Methods The models of PCSK9 (RNAi-PC-SK9) and PCSK9 (0E-PCSK9) were constructed on HepG2 and L02 cells by using the existing lentivirus in laboratory. The ratio of lentivirus infection was detected by inverted fluorescence microscopy. The effect of overexpression and RNA interference PCSK9 was detected by Western blot; lipid depositional effect of curcumin on cells was detected by oil red O staining analysis, and cholesterol levels in cells were ana...