期刊论文

Effects of Jiawei Danshen Yin combined with MiR-21 on IRI myocardial cells

中文

中国药理学通报

1001-1978

2020

36

2

277-281

10.3969/j.issn.1001-1978.2020.02.024

国家自然科学基金青年科学基金项目(No 81704065) 湖南省教育厅优秀青年项目(No 16B198) 中医内科学省部共建教育部重点实验室开放基金重点项目(No ZYFT201507) 湖南省自然科学基金项目(No 2017JJ3239,No 2019JJ40225) 湖南省中医药科研计划课题项目(No 201790)。

本校为第一机构

目的探讨加味丹参饮联合miR-21对IRI心肌细胞是否有保护作用,并研究其保护机制。方法培养H9C2心肌细胞,分为四组:空白组,心肌细胞IRI模型组,miR-21组,miR-21联合加味丹参饮组(JWDSY组),检测心肌细胞的凋亡率、凋亡相关蛋白和PTEN信号通路表达情况。结果电镜可见模型组细胞线粒体明显受损,JWDSY组细胞超微结构损伤程度减轻;ELISA法检测模型组cTnl升高,JWDSY组降低,差异有统计学意义(P

Objective To investigate the protective effect of Jiawei Danshen Yin combined with microRNA-21 on IRI myocardial cells,and to study its protective mechanism. Methods H9C2 cardiomyocytes were cultured and divided into four groups: Blank group, IRI model group of cardiomyocytes,miR-21 group,miR-21 combined with Jiawei Danshen Yin group (JWDSY group). Apoptotic rate, apoptosis-related protein and PTEN signaling pathway expression of cardiomyocytes were detected. Results Electron microscopy showed that mitochondria in model group were damaged signi...