期刊论文

Polarization of microglia in cortex of MCAO rats and intervention of Naotaifang Ⅱ

中文

中国药理学通报

1001-1978

2020

36

9

1298-1303

10.3969/j.issn.1001-1978.2020.09.021

国家自然科学青年基金资助项目（No 81603608,81774174）； 教育部博士后面上项目一等资助项目（No 2018M630905）； 湖南省自然科学青年基金资助项目（No 2019JJ50431）； 湖南中医药大学中医学一流学科开放基金资助项目（No 4901-020000200221）； 湖南省大学生研究性学习和创新性实验计划项目（No 1021-0001017161）； 中医内科重大疾病防治研究与转化教育部重点实验室开放基金资助项目（No ZYNK201708）；

本校为第一机构

目的探讨局灶性脑缺血（middle cerebral artery occlusion,MCAO）大鼠皮质小胶质细胞（microglia,MG）极化的变化规律,揭示脑泰方Ⅱ号调节MG极化的方式与特点。方法将SD大鼠随机分为正常组、假手术组、MCAO模型组、米诺环素组、脑泰方Ⅱ号组,于造模后d 1、3、5、7激光共聚焦观察MG形态;Real time-PCR法检测M1/M2型表面标记分子表达。结果 MCAO模型组MG胞体胀大,突触变粗、短,分支减少;脑泰方Ⅱ号可明显促进突触分支与MG增殖。模型组M1/M2型标志分子随时间被不同程度活化;脑泰方Ⅱ号自d 3后（米诺环素自d 1后）可逐步明显抑制M1型标记分子,促进M2型标记分子表达（P

Aim To explore the changing rule of microglia polarization in MCAO rat cortex, and to reveal the action mode and characteristics of Naotaifang II on microglia polarization. Methods SD rats were randomly divided into five groups; normal group, shamoperated group, MCAO model group, minocycline group and Naotaifang II group. On the 1st, 3rd, 5th and 7th day after the establishment of model, laser confocal scanning was used to observe the morphology of microglia, Real-time PCR to detect the expression of M1/M2 surface markers. Results In model grou...