期刊论文、会议论文

Wu Na

中文

化学学报

0567-7351

2009

67

7

700-704

第三届中医药现代化国际科技大会

第三届中医药现代化国际科技大会论文集

2010-11-25

成都

中华中医药学会<&wdkj&>科技部<&wdkj&>卫生部

10.3321/j.issn:0567-7351.2009.07.020

本校为其他机构

将巴马火麻仁木脂素酰胺类的粗提物采用硅胶柱层析法以V(氯仿):V(甲醇)=85:15洗脱分离,分离产物经快原子轰击质谱(FAB-MS)、核磁共振(NMR)鉴定为人麻酰胺A(cannabisin A).以1,1-二苯基-2-苦基肼自由基(DPPH)、超氧阴离子自由基(O_2~-)、羟自由基(OH~·)三种不同自由基体系研究火麻仁木脂素酰胺类的粗提物、大孔树脂纯化后的精提物及cannabisin A的活性清除作用.结果表明在一定的剂量范围内,三者均有显著的活性清除作用,且与剂量呈明显量效关系.精提物的清除作用最强,尤其是在OH~·体系中,其清除活性为粗提物的13倍.

Cannabisin A was isolated from the crude lignanamides of Fructus Cannabis of Bama with chloroform-methanol (85:15, V:V) on silica gel column chromatography, and identified by FAB-MS and NMR. The scavenging activity of the crude lignanamides, fine lignanamides purified by macroporous resin and cannabisin A was studied by three different free radical systems such as 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH ·), superoxide anion free radical (O2-) and hydroxide free radical (OH ·). The results showed that all of them had significant scav...