期刊论文

中文

中西医结合心血管病电子杂志

2095-6681

2018

6

36

5-8

10.16282/j.cnki.cn11-9336/r.2018.36.004

81473615:国家自然科学基金 2015JJ2017)(2015JJ2017:湖南省自然基金项目

本校为第一机构

目的探讨TLR4-/-小鼠动脉粥样硬化时β-catenin的变化及护心康的干预作用。方法 SPF级TLR4-/-小鼠共70只,随机取12只为对照组,予以普通饲料喂养;其余58只予以高脂饲料造模,喂养13周后从造模动物中随机选取5只解剖观察其胸主动脉,光镜下发现粥样斑块为造模成功。造模成功动物随机分为模型组、护心康组、阿托伐他汀组,Wnt抑制剂WIF组,每组12只,分别予以相应处理。治疗八周后处死小鼠,心脏采血,酶比色法检测血脂;取胸主动脉,光镜下观察动脉粥样硬化形成情况;免疫组化法检测斑块中β-catenin蛋白的表达。结果 （1）血脂检测造模后动物胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白水平比正常组明显...

Objective To investigate the changes ofβ-catenin and the intervention of Huxinkang in atherosclerosis.Methods70SPF TLR4-/-mice were fed,and12mice of them were randomly selected as control group and fed with normal diet.The remaining58mice were modeled with high-fat diet.After13weeks of feeding,5mice were randomly selected from them to observe the thoracic aorta,and atheroma plaque was found under light microscopy indicating successful modeling.The successful model animals were randomly divided into model group,Huxin Kang group,atorvastatin gro...