期刊论文

Influence of Ziyin Huoxue Jiedu Decoction on Expression of Adhesion Molecules and Blood Coagulation Related Factors in Vascular Endothelial Cells Induced by AdvancedGlycation End-Products

中文

湖南中医药大学学报

1674-070X

2017

37

5

485-488

10.3969/j.issn.1674-070X.2017.05.006

14JJ2113:湖南省自然科学基金 201606:湖南省中医药科研项目 16K063:湖南省教育厅项目 :中西医结合基础湖南省重点学科建设项目

本校为第一机构

目的 观察滋阴活血解毒方对糖基化终末产物(advanced glycation end-producs,AGEs)诱导血管内皮细胞(vas-cular endothelial cell,VEC)损伤的影响及其黏附分子、凝血相关因子表达的变化,进一步探讨该方对AGEs诱导VEC损伤的保护作用.方法 以人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)作为实验对象,以终浓度(100 mg/L)的AGEs诱导HUVEC损伤.各模型组及给药组细胞分别继续培养12、24、48 h.在显微镜下观察各组细胞形态变化,并以Western-blot法检测各组黏附分子VCAM-1、ICAM-1的表达;以RT-PCR方法 检测TF、TM mRNA表达.结果 相对于空白组细胞,模型组细胞出现异常增殖,细胞数量...

Objective To investigate the influence of Ziyin Huoxue Jiedu decoction on the expression of adhe-sion molecules and blood coagulation related factors in the vascular endothelial cells (VEC) induced by advanced glycation end-products (AGEs). Methods The human umbilical vein endothelial cell as the subjects, the human umbilical vein endothe-lial cells (HUVEC) damage were induced by AGEs with the final concentration (100 mg/L), and the cells were continually cultured for 12, 24, 48 h. The cell morphological changes were observed, the dhesion molecule VCAM-1 and ICAM-1 ex-pression were determined ...