期刊论文

Effect mechanism of Yanghe decoction on bone metastasis of human breast cancer xenografts in nude mice

中文

湖南中医药大学学报

1674-070X

2012

32

7

27-30

10.3969/j.issn.1674-070X.2012.07.008.027.04

09K058:湖南省高等学校创新平台开放基金 20114323110006:高等学校博士学科点专项科研基金 07C488:湖南省教育厅项目

本校为第一机构

目的 探讨阳和汤对裸鼠移植性人乳腺癌骨转移模型的作用及其相关调节因子的影响.方法 股骨接种人乳腺癌细胞建立裸鼠乳腺癌骨转移模型.随机分为阳和汤高、低剂量组、唑来磷酸组、模型组、假手术组,动态观察各组裸鼠骨转移癌的生长情况,测量骨转移癌体积,绘制生长曲线;给药20d后处死动物,测取瘤质量,计算抑瘤率;ELISA法检测裸鼠血清中相关调节因子PTHrP、RANKL、OPG含量的变化.结果 1.阳和汤高、低剂量组、唑来磷酸组的骨转移癌灶体积均小于模型组,差异有统计学意义(P＜0.05);2.阳和汤高、低剂量组、唑来磷酸组PTHrP和RANKL含量明显低于模型组,差异有统计学意义(P＜0.05),而OPC含量水平增加,RANK...

Objective To study the breast cancer xenografts The model of bone metas inoculating effects of Yanghe decoction on bone in nude mice and the impacts in relevant regulato metastasis ry factors. of human Methods tasis of human breast cancer xenografts in nude mice was established by breast cancer cells in femur. These mice were as follows： Yanghe decoction high -dose group and randomly divided into 5 groups low -dose group, zoledronic acid group, model group and sham-operated group. Bone metastasis tumors of different groups were observed dynami...