期刊论文

Comparison of Two Lactase Activity Determination Methods

Huaying, H.

中文

应用化学

1000-0518

2020

37

2

242-244

10.11944/j.issn.1000-0518.2020.02.190215

国家自然科学基金青年基金（81703985）项目资助~~；

本校为第一机构

邻硝基苯-β-D-吡喃半乳糖苷( ONPG)法和双糖酶法是常用的乳糖酶活力测定方法,本文对这两种方法的线性范围、检出限、精密度、准确度和稳定性进行了比较。结果显示,ONPG法在线性范围、检出限、精密度以及稳定性方面均优于双糖酶法,但此种方法的重复性较差,需要进一步的优化。对比这两种方法,可为乳糖酶活力测定方法的合理选取提供参考。

Both o-nitrophenyl β-D-galactopyranoside(ONPG) method and disaccharase method are commonly used for the determination of lactase activity. This paper compared the linear range, detection limit, precision, accuracy and stability of these two methods. It is found that ONPG method is better than disaccharase method in linear range, detection limit, precision and stability, but this method has poor repeatability and needs further optimization. ©...