期刊论文

Yi Ning<&wdkj&>Fangguo Lu

英文

Talanta

0039-9140

2022

250

123714

10.1016/j.talanta.2022.123714

National Natural Science Foundation [81803964, 82074250, 81973670]; Xinglin talent cultivation program; Natural Science Foundation of Hunan Province [[2019] 370-118, 2020JJ4465, 2020JJ4063]; Key Project of the Department of Education of Hunan Province [2020JJ5418]; Research and Innovation Program for postgraduates in Hunan Province [21A0237]; Western medicine in Hunan Province, Basic medicine construction project of Hunan University of traditional Chinese Medicine [6]; first-class cultivation subject of integrated Chinese and Western medicine in Hunan Province; Basic medicine construction project of Hunan University of traditional Chinese Medicine [CX20210700]; Excellent Teaching Team of Postgraduate in Hunan Province [6]; Hunan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine [[2019] 370-118]; [2019TP1044]

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Herein, we report the development of a graphene-oxide-based (GO-based) fluorescent bioassay for determining agrC gene transcription (mRNA) in methicillin-resistant Staphylococcus aureus (MRSA). The design is based on nicking-enzyme-assisted (Nb.BbvcI-assisted) target recycling amplification (NATR) and a hybridization chain reaction (HCR). The system consists of a helper probe (HP), a molecular beacon (MB) probe, four hairpins, and endonuclease Nb.BbvcI, which plays a role in target recycling and signal amplification. In the absence of the targe...