Online coupling solid-phase ligand-fishing with high-performance liquid chromatography-diode array detector-tandem mass spectrometry for rapid screening and identification of xanthine oxidase inhibitors in natural products
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21275163]; Science and Technology Program of Hunan Province, China [2015NK3037]; Shenghua Yuying project of Central South University
机构署名：
本校为通讯机构
摘要：
Screening and analysis of bioactive compounds from natural products is challenging work due to their complexity. This study presents the first report on hyphenation of solid-phase ligand-fishing using immobilized xanthine oxidase microcolumn (IXOM) and high-performance liquid chromatography–diode array detector–tandem mass spectrometry (HPLC–DAD–MS/MS) for screening and identification of XO inhibitors from complex mixtures. Solid-phase ligand-fishing system was hyphenated with the HPLC system via four-port switching valve and a six-port injection valve as an interf...
