Dual-signal ratiometric electrochemical aptasensor for Zearalenone detection based on magnetic-assisted enrichment and hybridization chain reaction

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成果类型：

期刊论文

作者：

Guo, Wentao;Zhang, Xuxin;Deng, Bin;Chen, Hao;Wu, Shun;...

通讯作者：

Wang, YH;Ning, G

作者机构：

[Wu, Yaohui; Guo, Wentao; Deng, Bin; Wang, Yonghong; Zhang, Xuxin; Chen, Hao; Wu, Shun] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol & Int Coope, Base Sci & Technol Innovat Forest Resource Biotech, Changsha 410004, Peoples R China.

[Wang, Yonghong] Yuelushan Lab, Changsha 410004, Peoples R China.

[Ning, Ge; Ning, G] Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Peoples R China.

通讯机构：

[Wang, YH ] C

[Ning, G ] H

Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol & Int Coope, Base Sci & Technol Innovat Forest Resource Biotech, Changsha 410004, Peoples R China.

Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Peoples R China.

语种：

英文

关键词：

Aptamer;HCR;Magnetic beads;Ratiometric aptasensor;Zearalenone

期刊：

Food Chemistry

ISSN：

0308-8146

年：

2025

卷：

465

期：

Pt 1

页码：

141963

DOI：

10.1016/j.foodchem.2024.141963

基金类别：

CRediT authorship contribution statement Wentao Guo: Writing – original draft, Investigation. Xuxin Zhang: Writing – original draft. Bin Deng: Validation, Software. Hao Chen: Validation, Software. Shun Wu: Writing – review & editing. Yaohui Wu: Writing – review & editing. Yonghong Wang: Supervision, acquisition. Ge Ning: Supervision, acquisition.

机构署名：

本校为通讯机构

院系归属：

国际教育学院

摘要：

In this work, a dual-signal ratiometric electrochemical aptasensor based on the hybrid chain reaction (HCR) and streptavidin-modified magnetic beads (SA-MBs) was developed to rapidly detect zearalenone (ZEN). The HCR, as a powerful signal amplification technique to imporve the signal of sonser. When the target is present, they specifically bind with ZEN-Apt and release ZEN-cDNA to trigger HCR. Simultaneously, more double-stranded DNA causes the signal of Thi to be blocked. As a result, the two signals tend to change in the opposite direction as...

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Dual-signal ratiometric electrochemical aptasensor for Zearalenone detection based on magnetic-assisted enrichment and hybridization chain reaction

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