期刊论文

Molecular Diagnostic Model of Saliva Protein Fingerprints in Gastric Cancer Patients with Spleen Deficiency

中文

中医杂志

1001-1668

2016

57

22

1949-1953

10.13288/j.11-2166/r.2016.22.015

国家自然科学基金（81273665） 湖南中医药大学中医诊断学国家重点学科开放基金（201401）

本校为第一机构

目的研究建立胃癌脾虚证唾液蛋白质指纹图谱新型分子诊断模型。方法采集未经手术和放化疗治疗的57例胃癌患者(脾虚证组40例,非脾虚证组17例)、45例健康志愿者(正常对照组)的唾液标本,用弱阳离子交换型(WCX)纳米磁珠联合基质辅助激光解析离子化飞行时间质谱(MALDI-TOF MS)技术进行检测,获得各组标本的蛋白指纹图谱。比较各组唾液蛋白质质谱数据,找出胃癌脾虚证组与正常对照组、胃癌脾虚证组与胃癌非脾虚证组之间具有统计学意义的差异表达蛋白质峰;采用决策树算法计算出多个变量即质荷比(m/z)变化对两组样本的判别价值,确定最优化的诊断模型。结果胃癌脾虚证组与正常对照组唾液蛋白质质谱比较共有...

Objective To study and establish a new molecular diagnostic model of saliva protein fingerprints in gastic cancer patients with spleen deficiency.Methods Saliva samples of 57 gastric cancer patients with no surgery and chemoradiotherapy (40 cases of spleen deficiency, 17 non-spleen deficiency) and 45 healthy volunteers were collected and tested by weak cation exchange (WCX) magnetic nanoparticles combined with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) to get the protein fingerprints of each group. Then the data were compared to figure out the d...