期刊论文

中文

海峡药学

1006-3765

2012

24

11

22-25

10.3969/j.issn.1006-3765.2012.11.008

基金项目：国家药典委员会国家药品标准提高行动计划项目（编号：223）

本校为第一机构

目的 提高完善藤黄健骨片质量标准.方法 采用显微法对淫羊藿和鹿衔草进行鉴别;采用TLC法对熟地黄,淫羊藿进行鉴别;采用HPLC法分别测定骨碎补中柚皮苷以及淫羊藿中淫羊藿苷的含量,色谱柱为ECOSIL C18(250mm×4.6mm×5μm),流动相分别为乙腈-水(15∶85),乙腈-水(30∶70);柱温35℃,流速为1.0mL·min-1,检测波长分别为283nm,270nm.结果 淫羊藿和鹿衔草的显微特征较易见,TLC色谱斑点清晰,专属性强,重复性良好.柚皮苷对照品在0.1364～3.41μg范围内线性关系良好(r2=0.9991),平均回收率100.17%,RSD为0.5%(n=6).淫羊藿苷对照品在0.04472～2.236μg范围内线性关系良好(r2=0.9999),平均回收率100.07%,RSD为...

OBJECTIVE To improve the quality standards of Teng Huang Jian Gu Tablets.METHODS Microsurgical method was used to identify Epimedium and Pyrolae.TLC method was used to identify rehmannia and Epimedium;HPLC method was used to determine the content of Naringin in Drynaria and the content of Icariin in Epimedium respectively,column ECOSIL C18（250mm×4.6mm×5μm） was eluted with mobile phase of acetonitrile-water（15∶85）,acetonitrile-water（30∶70）,at column temperature 35℃,The flow rate was 1.0mL·min-1 and detective wavelength was 283nm,270...