期刊论文

The Antineoplastic Activity Screening of Different Extracts from Clerodendrum bungei Steud and Their Effects on EMT-Related Proteins in Nude Mice with Transplanted Tumor

中文

中药药理与临床

1001-859X

2020

36

04

124-131

10.13412/j.cnki.zyyl.2020.04.012

中国博士后基金面上项目（编号:2019M652775）； 湖南省中医药管理局课题（编号:201935）； 中药学湖南省重点学科开放基（编号:zy201602）； 湖南省大学生创新项目（编号:306）；

本校为第一机构

目的:比较臭牡丹不同提取部位的抗肿瘤作用及其作用机制。方法:根据前期研究结果,采用乙酸乙酯、石油醚萃取、大孔树脂纯化方法,将臭牡丹苯乙醇苷类、黄酮类、萜类成分进行分离提取并纯化。建立CCK8、划痕试验、Transwell侵袭小室经典的抗肿瘤药理模型,检测臭牡丹三大活性部位对人肺腺癌A549体外增殖、迁移、侵袭的影响。并通过建立A549裸鼠移植瘤模型,检测各组裸鼠癌组织与癌旁组织中β-连环蛋白(β-catenin) 、波形蛋白(Vimentin) 、上皮细胞钙粘蛋白(E-cadherin) 、糖原合成酶激酶-3(GSK-3β) 、磷酸化糖原合成酶激酶-3蛋白(p-GSK-3β)蛋白的表达水平。结果:苯乙醇苷部位以类叶升麻苷和异类叶...

Objective: To investigate the antitumor effect of different extracts from Clerodendrum bungei Steud and their mechanism. Methods: According to the previous study results,the phenylethanol glycosides,flavonoids and terpenes of Clerodendrum bungei Steud were extracted by ethyl acetate,petroleum ether and purified by macroporous resin. Classical anti-tumor models like CCK8 assay, the scratch test and Transwell invasion models were established,the effects of three active parts from Clerodendrum bungei Steud on proliferation,migration and invasion o...