期刊论文

Effects of Huluan Decoction on ovary cell the total live fetus of rats with GnRHa ovarian hyperstimulation apoptosis and controlled

中文

湖南中医药大学学报

1674-070X

2013

33

1

99-101,117

10.3969/j.issn.1674-070X.2013.01.028.099.03

湖南省教育厅资助项目（10C1043）； 湖南省高校科技创新团队项目资助； 湖南省高校重点学科中西医结合基础项目资助；

本校为第一机构

目的 观察护卵汤对促性腺激素释放激素(GnRHa)超排卵大鼠卵巢细胞凋亡及活胎数的影响,为护卵汤辅治辅助生殖技术提供实验依据.方法 模仿人类辅助生殖中的长周期,对大鼠进行GnRHa垂体降调节预处理后再用尿促性腺激素(HMG)等药物超排卵(下称模型组),同时给予不同剂量的护卵汤辅助治疗,以及自然周期未使用任何药物的大鼠为正常对照(下称正常组)在注射绒毛膜促性腺激素(HCG)当天摘取卵巢组织用缺口末端标记法(TUNEL法)检测卵巢细胞凋亡情况;在孕12 d观察各组大鼠的妊娠胚胎数.结果 HCG日模型组卵巢体细胞凋亡较正常组显著增多,差异有统计学意义(P＜0.01);护卵汤各剂量组卵巢细胞凋亡均较模型组减少,...

Objective To observe the effects of Huluan Decoction on ovary cell apoptosis and the total live fetus of rats with GnRHa controlled ovarian hyperstimulation （COH）, and to provide related experimental evidence for the effectiveness of Huluan Decoction in assisted reproductive technology （ART）. Methods After GnRHa pituitary down regulation pretreatment which imitated the human long period of COH, a number of rats were separated into model group treated with HMG/HCG superovulation drugs and treatment groups treated with HMG/HCG superovulation ...