期刊论文

中文

中国医药导报

1673-7210

2017

14

17

19-22

81303003:国家自然科学基金 81403427:国家自然科学基金

本校为第一机构

目的 探讨慢性制动应激建立卵巢早衰及卵巢低反应小鼠模型的方法.方法 利用自制制动器对SPF级ICR雌性小鼠进行制动,造成慢性应激状态,观察小鼠的一般情况,称量体重,阴道涂片观察动情周期.制动完全结束后进行微刺激超排卵,在超排卵第3天和HCG注射后24 h计数各级卵泡.结果 与未制动小鼠相比,制动期间小鼠的进食、毛色等一般情况变差,体重明显减轻(P＜0.05),动情周期异常的例数明显增多(P＜0.05),但超排卵第3天和HCG后24h小鼠各级卵泡计数比较,制动小鼠与未制动小鼠没有明显区别(P＞ 0.05).结论 慢性制动应激可以引起小鼠卵巢功能紊乱或减退,但能否造成卵巢早衰及卵巢低反应还需进一步探讨.

Objective To explore the method of chronic immobilization stress which can establish the mice model of premature ovarian function (POF) and poor ovarian response (POR).Methods Self-made brakes were used to make chronic stress stateon SPFICR female mice,during the general situation,body weight and estrous cycle of these mice were observed.The microstimulated super ovulation was used on the mice after immobilization,and the follicles of the mice were counted respectively on the third day of superovulation and 24 h after HCG injection.Results Comp...