期刊论文

中文

中国医药导报

1673-7210

2017

14

4

16-19

国家自然科学基金（81503492）.

本校为第一机构

目的 优选骨刺康复丸的提取工艺参数,为该制剂工业化生产提供依据。方法 以浸膏得率与青藤碱含量为评价指标,采用流动相：乙腈-乙二胺（32∶68）,流速1.0 m L/min,检测波长262 nm;柱温30℃的HPLC Welchrom C18色谱柱（250 mm×4.6 mm,5μm）测定青藤碱含量,单因素试验考察药材吸水率、提取次数,正交设计试验法考察加水量、煎煮时间、浸泡时间三个因素在不同水平对实验结果的影响。结果 骨刺康复丸最佳水提工艺参数为：药材吸水率为180.9%,提取2次,第一次加10倍量水,提取2.5 h,第二次加8倍量水,提取2 h。按最佳水提工艺参数验证试验结果表明,浸膏得率可达33.08%、RSD为0.64%、青藤碱含量可达16.7...

Objective To optimize extraction technical parameters of Guci Kangfu pills, and to provide the evidence for industrial production. Methods The rate of extractum and sinomcnine contents were selected as evaluation index; Sinomenine content was determined with the mobile phase of acetonitrile-ethlenediamine （32：68）, the flow rate of 1.0 mL/min,detection wavelength 262nm,and HPLC welchrom C18 （250 mm×4.6 mm,5μm） with column temperature of 30℃. The water absorption rate and the number of extraction times were investigated by the single fact...