期刊论文

Guan, C.-X.

中文

中南大学学报(医学版)

1672-7347

2007

32

5

771-775

10.3321/j.issn:1672-7347.2007.05.009

基金项目：国家自然科学基金（39800053）

本校为其他机构

目的:探讨降钙素基因相关肽(calcitonin-gene-related peptide,CGRP)对人支气管上皮细胞分泌基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)/明胶酶B的影响及其机制.方法:采用逆转录聚合酶链式反应及明胶酶谱法,观察给予不同浓度的CGRP(10~(-10)～10~(-6) mol/L)刺激及CGRP 10-6mol/L刺激后不同时间点(6,12,18,24,36和48 h)MMP-9活性和基因表达量的变化.结果:体外培养的人支气管上皮细胞能分泌MMP-9;CGRP干预呈剂量及时间依赖方式上调人支气管上皮细胞MMP-9的活性和MMP-9 mRNA的表达(P＜0.01);CGRP上调人支气管上皮细胞MMP-9活性和表达的作用可为蛋白激酶C阻断剂H-7及钙调蛋白阻断剂W-7部...

OBJECTIVE: To examine the expression of matrix metalloproteinase-9 (MMP-9) in human bronchial epithelial cells treated with calcitonin-gene-related peptide (CGRP). METHODS: RT-PCR and gelatin zymography were performed to examine the dynamic expression and activity of MMP-9 in human bronchial epithelial cells at different doses (10(-10), 10(-9), 10(-8), 10(-7), and 10(-6)mol/L) and different time points (6,12,18,24,36, and 48h) after the stimulation of CGRP. RESULTS: The unstimulated human bronchial epithelial cells only secreted a small amount of MMP-9. After the CGRP stimulation, the expressi...