期刊论文

中文

解剖学杂志

1001-1633

2018

41

2

160-162

10.3969/j.issn.1001-1633.2018.02.008

湖南省教育厅优秀青年课题(16B269) 益阳医学高等专科学校引进***人才科研启动费(2016-001) 益阳市科技局课题(2015JZ42)。

本校为其他机构

目的:探讨健康大鼠侧脑室室管膜区(SVZ区)Sp8与微管相关蛋白Doublecortin (DCX)的表达.方法:1、6、12月龄SD大鼠被处死.脑冠状位切片,免疫荧光单标染色观察1、6、12月龄组SVZ区Sp8细胞与DCX细胞的表达,免疫荧光双标染色观察1月龄组Sp8/GFAP、Sp8/Ki67的共表达情况。结果月龄组SVZ区Sp8细胞、DCX细胞密度均显著高于6月龄组及12月龄组;除此之外,1月龄组SVZ区有Sp8/GFAP、Sp8/Ki67的共表达细胞.结论:Sp8与DCX在大鼠的SVZ区有广泛的表达;Sp8调控SVZ区神经发生和星形胶质干细胞的分化.

Objective: To explore the expression of Sp8 and DCX in the subventricle zone (SVZ) of lateral ventricle in healthy adult rats. Methods : Sprague Dawley rats aged 1,6 and 12 months were sacrificed and Brain specimens were sectioned with coronal position. The expression of the Sp8 and DCX in the SVZ in 1-month group, 6-month group and 12-month group was assessed by immunofluorescence single labelling and the expression of Sp8/GFAP, Sp8/Ki67 in the SVZ in 1-month group was detected by immunofluorescence double labelling. Results : The cell density of the Sp8 cells and DCX cells in the SVZ at 1-mo...