期刊论文

中文

山东理工大学学报(自然科学版)

1672-6197

2019

33

4

74-78

10.3969/j.issn.1672-6197.2019.04.016

哈尔滨商业大学研究生创新科研项目(YJSCX2018-546HSD).

本校为其他机构

对正品枳及其5种常见混伪品进行ITS2分析,为制药和临床上鉴别枳实和枳壳类中药材提供一种方法,也为农业上鉴定橘类种子种苗提供一种可靠方法。提取枳及其混伪品DNA,对ITS2序列扩增和双向测序,从GenBank下载其他部分序列。用DNAstar 7.10进行序列长度统计,并计算G+C含量。用MEGA 5.0计算种内种间(K2P)遗传距离,并进行NJ树聚类分析和二级结构分析,最后通过条形码网站鉴定各物种序列。结果发现:枳及其混伪品ITS2序列长度范围为231~279bp,G+C含量范围为69.26%~72.93%;种内最大K2P遗传距离比种间最小遗传距离小;聚类树单系性良好,但二级结构区分不明显,条形码网站鉴定可得到正确物种。ITS2条形码可有...

We identify Citrus aurantium L. and its adulterants using ITS2 sequence to provide a secure and accurate identification way for the pharmaceutical and clinical use of Citrus aurantium L.,and also to provide a reliable identification method of citrus seeds or seedlings for agriculture.After extracting the DNA of Citrus aurantium L and its adulterants, the ITS2 sequences were amplified by PCR and measured bidirectionally;and the other sequences were downloaded from GenBank. DNAstar 7.10 is used to measure the length of each ITS2 sequence and calculate the G+C content of each ITS2 sequence.The ge...