期刊论文

Effect of Procyanidin in Promoting Apoptosis of MDA-MB-231 Triple-Negative Breast Cancer Cell Lines

中文

湖南中医药大学学报

1674-070X

2017

37

11

1196-1199

10.3969/j.issn.1674-070X.2017.11.007

湖南省教育厅课题（13C691）；

本校为第一机构

目的 研究原花青素(Procyanidins,PC)对三阴乳腺癌MDA-MB-231细胞株增殖及细胞凋亡的作用及其机制.方法 常规培养细胞,24 h后随机分为阳性对照组、 对照组和PC10、20、40、80、160、320μg/mL组.MTT法检测细胞生长抑制率;流式细胞术检测细胞凋亡;Western blot法检测FoxA1蛋白及抗凋亡蛋白bcl2、UCP2表达.结果 PC各剂量组均可显著抑制抑制MDA-MB-231细胞增殖(P

Objective To study the effect and mechanism of procyanidins (PC) on the proliferation and apoptosis of MDA-MB-231 breast cancer cell line. Methods The cells were randomly divided into positive control group, control group and PC (10, 20, 4., 80, 160, 320 μg/mL) groups. Growth inhibiting rate of MDA-MB-231 cell was detected by MTT assay. Apoptosis was detected by flow cytometry, and the expression of FoxA1 protein and anti apoptotic protein (UCP2 and bcl2) were detected by Western blot. Results Different doses of PC could infinicantly inhibited...