期刊论文

中文

中国实验动物学报

1005-4847

2017

25

1

70-73

10.3969/j.issn.1005-4847.2017.01.013

湖南省教育厅重点项目（编号：15A140） 湖南中医药大学研究生科研创新课题（编号：2016CX16）

本校为第一机构

目的建立氢化可的松致肾阳虚、肾阴虚小鼠模型,并探讨其相关评价指标。方法采用氢化可的松灌胃建立肾阳虚及肾阴虚小鼠模型;观察肾阳虚、肾阴虚模型小鼠生存状态,测定各组模型小鼠体重变化;采用放射免疫法检测HPA轴中促肾上腺皮质激素(ACTH) 、皮质醇(Cor.) 、HPT轴中甲状腺激素(TSH) 、三碘甲腺原氨酸(T3) 、甲状腺素(T4) 、HPG轴中促卵泡素(FSH) 、雌二醇(E2) 、睾酮(T)含量。结果肾阳虚、肾阴虚模型小鼠体重均下降,肾阳虚模型小鼠血清ACTH、Cor. 、TSH、T3、T4水平降低,血清FSH、E2、T水平升高,且差异均有显著性(P < 0.01);而肾阴虚模型则反之。结论肾虚小鼠内分泌系统中ACTH、Cor. 、TSH、T...

Objective To establish a mouse model of kidney-yin and kidney?yang deficiency after oral administra?tion of hydrocortisone, and to explore the related evaluation factors. Methods The model was established by oral adminis?tration of hydrocortisone to induce kidney?yin and kidney?yang deficiency in mice. The survival and body weight of the mice were observed. The serum content of adrenal cortical hormone ( ACTH) , cortisol ( Cor. ) in the hypothalamic?pituitary?ad?renal (HPA) axis, and thyroid stimulating hormone (TSH), triiodothyronine (T3), thyrox (T4) in the hypothalamic?pi?tuitary?thyroid (H...