期刊论文

Construction and evaluation of two obese rat models of polycystic ovary syndrome with insulin resistance

中文

中国实验动物学报

1005-4847

2021

29

05

644-650

10.3969/j.issn.1005-4847.2021.05.011

2019JJ50350:湖南省自然科学基金 2019JJ50345:湖南省自然科学基金 201941:湖南省中医药科研项目 201803:湖南省中医药研究院重点项目

本校为第一机构

目的采用两种不同方式联合高脂饮食建立肥胖型多囊卵巢综合征伴胰岛素抵抗(PCOS-IR)大鼠动物模型,比较其内分泌及代谢特征变化。方法将大鼠随机分为3组:对照组,给予普通饲料和纯净水;Letrozole组,采用来曲唑(1 mg/kg)灌胃,HCG+INS组,皮下注射绒促性素和胰岛素(HCG+INS),两组均采用高脂饲料和5%葡糖糖液饲养。革兰氏染色观察两个动情周期变化;在第23天和第30天,测量子宫和卵巢湿重;苏木素-伊红(HE)染色观察卵巢组织病理学改变;血液生化仪测定空腹血糖(FBG)、血脂(甘油三酯(TG)和总胆固醇(TC))变化;酶联免疫吸附(ELISA)法测定性激素(卵泡刺激素FSH、促黄体生成素(LH)、雌二醇(E_(2))、总睾酮(T)、...

Objective To establish an animal model of obese polycystic ovary syndrome combined with insulin resistance (PCOS-IR) using two different method with a high-fat diet,and to compare the changes in endocrine and metabolic characteristics.Methods Rats were randomly divided into a control group (ordinary feed and purified water),a letrozole group (intragastric administration of 1 mg/kg),and a human chorionic gonadotropin + insulin (HCG+INS) group(subcutaneous injections).The latter two groups were fed a high-fat diet and 5% glucose solution.Gram sta...