期刊论文

Comparison of different regimens in isoprenaline-induced chronic heart failure models

中文

中国实验动物学报

1005-4847

2024

32

2

161-167

10.3969/j.issn.1005-4847.2024.02.003

本校为第一机构

目的 比较3种不同方案制备的动物模型,探索稳定、可靠且重复性好的小鼠慢性心力衰竭模型.方法 将25只雄性C57BL/6J小鼠随机分为4组:正常组(ZC组)、实验A组(MA组)、B组(MB组)和C组(MC组).实验组采取不同制备方案连续注射ISO,其中MA组和MB组为浓度递减造模法,MA组(第1天10 mg/kg、第2 天 5 mg/kg、第 3～30 天 2.5 mg/(kg·d);皮下注射 30 d);MB 组(第 1 天 20 mg/kg、第 2 天 10 mg/kg、第 3～14 天5 mg/(kg·d);皮下注射14d);MC组(浓度恒定7.5 mg/(kg·d),腹腔注射28 d),构建慢性心衰动物模型.在注射结束后的第2天,计算各组小鼠存活率和成模率情况.通过心脏超声检测心功能,并用ELISA测定血清中N...

Objective To compare animal models of chronic heart failure(CHF)prepared by three different protocols,to establish a stable,reliable,and reproducible mouse model of CHF.Methods Twenty-five male C57BL/6J mice were divided randomly into four groups:a blank group,model A group(MA group),model B group(MB group),and model C group(MC group).The model groups adopted different preparation protocols for continuous injection of isoprenaline.The MA group and MB group were dose-decreasing models:MA group:subcutaneous injection of 10 mg/kg on day 1,5 mg/kg ...