期刊论文

Effects of thin recipe of Buyang Huanwu Decoction on angiogenesis and signal pathway of Nrf2/HO-1 after cerebral ischemic injury in rat

中文

中国药理学通报

1001-1978

2016

32

1

123-128

10.3969/j.issn.1001-1978.2016.01.026

国家重点基础研究发展计划（973计划）项目（No2012CB723503） 湖南省科学技术厅科技计划一般项目（No2013SK3107） 湖南省中医内科学省部共建教育部重点实验室开放基金（NoZYNK201406）

本校为第一机构

目的：观察补阳还五汤精简方对大鼠脑缺血后血管新生及核转录因子 E2相关因子2( Nrf2)/血红素加氧酶-1(HO-1)信号途径的影响。方法120只 SD 大鼠随机分成假手术组、模型组、补阳还五汤组、补阳还五汤精简方组。采用大脑中动脉线栓法建立局灶性脑缺血模型,各组给予相应处理。在 d 1、3、7三个时间点取脑组织进行检测,免疫组织化学法检测血浆Ⅷ因子相关抗原(vWF),测定微血管密度(MVD)。采用 Real-time PCR 和 Western blot 法检测大鼠脑组织中 Nrf2、HO-1基因及蛋白表达。结果①与假手术组比,模型组 vWF 阳性表达微血管在 d 3时表达明显升高(P

Aim: To observe the effects of thin recipe of Buyang Huanwu Decoction on angiogenesis and the signal pathway of Nrf2/HO-1 after cerebral ischemic injury in rats. Methods: Totally 120 SD rats were randomly divided into four groups; sham operation group, model group, Buyang Huanwu Decoction group and thin recipe of Buyang Huanwu Decoction group. The focal cerebral ischemia rat model was established by middle cerebral arterial occlusion. Each group was treated with corresponding treatment. Each group was detected after cerebral ischemia for day 1, day 3 and day 7, respectively. Immunohistochemica...