期刊论文

Liu, Hairong(liuhairong@hnu.edu.cn)

中文

湖南大学学报(自然科学版)

1674-2974

2017

44

6

87-95

10.16339/j.cnki.hdxbzkb.2017.06.015

教育部新世纪优秀人才（NECT-12-0170）； 湖南省自然科学基金（2016JJ2023）； 湖南省研究生创新基地（2014-2017）； 湖南大学交叉学科研究资助项目（2014JCA09）~~；

本校为其他机构

组织工程支架材料表面性质对细胞的黏附起着重要作用,进而影响细胞的增殖、分化等一系列生长过程.本研究采用天然生物大分子透明质酸(hyaluronic acid,HA)对聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(Poly(3-hydroxybutyrate-co-3-hydroxyvalerate),PHBV)进行表面修饰以提高材料的表面生物活性.首先通过静电纺丝法制备PHBV纳米纤维支架,利用1,6-己二胺胺解在PHBV表面引入自由胺基,并以此为反应活性位点在水溶性的碳化二亚胺/N-羟基琥珀酰亚胺缩合剂体系中将透明质酸固定接枝在PHBV表面.SEM结果表明,静电纺丝制备的PHBV纳米纤维支架表面平滑程度高,纤维直径分布较均匀,且没有串珠;FTIR证明1,6-己二胺改性...

Surface properties of tissue engineering scaffold play important roles in cell adhesion and other biological processes such as cell proliferation, differentiation. In this study, the surface modification of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was performed in order to improve the biological activity of material surface. PHBV nanofibrous scaffold was firstly made by electrostatic spinning method, introducing free amino groups in the PHBV surface through 1, 6-hexanediamine, which provided reaction sites for the further immobilization of hyaluronic acid (HA) macromolecules. The m...