Guilu Erxian glue mitigates oxidative damage in mouse GC-1 spermatogonial cells by inhibiting autophagy via the Keap1/Nrf2 pathway

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成果类型：

期刊论文

作者：

Jin Ding;Wen Sheng;Wei Fu;Meixin Lin;Bonan Li;...

通讯作者：

Xing Zhou<&wdkj&>Qinghu He

作者机构：

School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 430000, China

Andrology Laboratory, Hunan University of Chinese Medicine, Changsha, 430000, China

[Wei Fu; Meixin Lin] Department of Andrology Clinic, Affiliated Bao'an Hospital of Traditional Chinese Medicine, The Seventh Clinical Medical School, Guangzhou University of Chinese Medicine, Shenzhen, 518133, China

Hunan University of Medicine, Huaihua, 418000, China

[Jin Ding] School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 430000, China<&wdkj&>Andrology Laboratory, Hunan University of Chinese Medicine, Changsha, 430000, China<&wdkj&>Department of Andrology Clinic, Affiliated Bao'an Hospital of Traditional Chinese Medicine, The Seventh Clinical Medical School, Guangzhou University of Chinese Medicine, Shenzhen, 518133, China

通讯机构：

[Xing Zhou; Qinghu He] S

School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 430000, China<&wdkj&>Andrology Laboratory, Hunan University of Chinese Medicine, Changsha, 430000, China<&wdkj&>School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 430000, China<&wdkj&>Andrology Laboratory, Hunan University of Chinese Medicine, Changsha, 430000, China<&wdkj&>Hunan University of Medicine, Huaihua, 418000, China

语种：

英文

关键词：

Male infertility;Oxidative damage;Traditional Chinese medicine;Guilu Erxian glue;Autophagy;Keap1/Nrf2 pathway

期刊：

中医科学杂志(英文)

ISSN：

2095-7548

年：

2023

卷：

期：

页码：

484-492

DOI：

10.1016/j.jtcms.2023.09.002

机构署名：

本校为第一且通讯机构

院系归属：

中西医结合学院

摘要：

To explore the effects and underlying mechanisms of Guilu Erxian glue (GLEXG) on oxidative damage in a mouse GC-1 spermatogonial (MGS) cell model. A cellular model for oxidative damage was created using MGS cells exposed to hydrogen peroxide (H2O2). Cell viability was assessed using the cell counting kit-8 assay, while reactive oxygen species (ROS) and malondialdehyde (MDA) levels were measured via flow cytometry and enzyme-linked immunosorbent assay, respectively. Western blotting and immunofluorescence techniques were employed to quantify the...

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Guilu Erxian glue mitigates oxidative damage in mouse GC-1 spermatogonial cells by inhibiting autophagy via the Keap1/Nrf2 pathway

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