期刊论文

Mechanism of Baicalin on the Apoptosis of Breast Cancer Cells by Up-Regulating miR-126

中文

湖南中医药大学学报

1674-070X

2017

37

5

481-484

10.3969/j.issn.1674-070X.2017.05.005

2015JJ6080:湖南省自然科学基金 15A139:湖南省教育厅重点项目 :湖南中医药大学青年教师科研基金

本校为第一机构

目的 用黄芩苷干预人乳腺癌细胞株MDA-MB-231,观察黄芩苷对乳腺癌细胞增殖、凋亡的影响及作用机制.方法 用qRT-PCR检测miR-126的表达变化,Western-blot检测Bcl-2、Caspase-9、Caspase-3、p-p38和p53的表达,MTT法检测细胞增殖,流式细胞术检测细胞凋亡.结果 miR-126在乳腺癌MDA-MB-231细胞中的表达比正常乳腺细胞低,黄芩苷干预乳腺癌细胞后miR-126上调最为明显(P

Objective To observe the effect and mechanisim of baicalin on proliferation and apoptosis of breast cancer cell line MDA-MB-231. Methods The expression of miR-126 was detected by qRT-PCR, and the expression of Bcl-2, Cas-pase-9, Caspase-3, p-p38, p53 was deternined by Western-blot. The cell proliferation was determined by using MTT method and cell apoptosis was detected by flow cytometry. Results The expression of miR-126 in breast cancer MDA-MB-231 cells was lower than that in normal breast cells, and the most obvious increase in miR-126 regul...