期刊论文

中文

中华中医药杂志

1673-1727

2016

31

12

5030-5034

湖南省高校科技创新团队项目 Funding Project of Preclinical Medicine and Pathogeny Biology of Key Discipline of Hunan University of Chinese Medicine Project of Education Department of Hunan Province 国家自然科学基金项目 Project of Traditional Chinese Medicine of Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province 湖南省教育厅项目 湖南中医药大学重点学科资助项目

本校为第一机构

目的:探讨仙茅多糖(COP)对免疫低下小鼠免疫功能的调节作用。方法:从72只昆明种小鼠中随机留取12只作为正常对照组,剩余60只采用环磷酰胺腹腔注射建立免疫低下模型,并随机分为模型对照组,COP高、中、低剂量组和阳性对照组,每组12只。COP高、中、低剂量组分别给予400、200、100mg·kg~(-1)·d~(-1)浓度的COP溶液灌胃,阳性对照组给予200mg·kg~(-1)·d~(-1)浓度的香菇多糖溶液灌胃,正常对照组和模型对照组给予等体积0.9%氯化钠溶液灌胃,每日灌胃1次,共灌胃处理10d。给药结束后观察小鼠免疫器官指数,流式细胞术检测血细胞中T细胞亚群,观察NK细胞杀伤能力和小鼠脾脏T、B细胞转化能力,用酶联免疫吸附...

Objective: To discuss the effect of Curculigo orchioides polysaccharide on the immunity of immunocompromised mice induced by cyclophosphamide. Methods: Twelve Kunming mice were selected randomly as the normal control group, and immunocompromised mice models were established in another 60 Kunming mice by intraperitoneal injection of cyclophosphamide. Then the mice models were randomly divided into 5 groups: model control group, positive control group, and Curculigo orchioides polysaccharide groups of low dosage, medium dosage, and high dosage. M...