期刊论文

中文

中南药学

1672-2981

2003

1

3

165-167

10.3969/j.issn.1672-2981.2003.03.015

本校为第一机构

目的建立细辛药材中马兜铃酸A的含量测定方法.方法HPLC法测定细辛药材中马兜铃酸A的含量.采用Spherigel C18高效液相色谱柱,以甲醇-水-醋酸(60:36:4)为流动相,检测波长316 nm.结果本方法线性关系良好(r=0.9995),平均回收率为97.7%,相对标准偏差RSD为0.50%(n=6).结论本方法简便,准确,回收率高,可用于细辛药材中马兜铃酸A的含量测定.

OBJECTIVE To establish a method to determine aristolochic acid A in herba asari. METHODS The condition of HPLC was: Spherigel C18 column, mobile phase with methanol-water-acetic acid (60 : 36 : 4), UV detector at 316 nm. RESULTS The calibration curve was linear (r=0. 9995). The average recovery was 97. 7%, and the RSD was 0. 50% (72 = 6). CONCLUSIONS The method is simple, and accurate with high high recovery rate, which is suitable for the de...