期刊论文

ITS sequence fingerprint of ultramicro Radix Puerariae

中文

中南药学

1672-2981

2007

5

2

173-179

10.3969/j.issn.1672-2981.2007.02.028

2001BA701A43:国家科技攻关计划 04C452:湖南省教育厅科学研究项目 24303:湖南省卫生厅中医药科研项目

本校为第一机构

目的 探求葛根超微饮片的品种鉴别方法.方法 以来自不同产地的4个野葛样品和10个粉葛样品制成的超微饮片为实验材料,用分子克隆方法获得并测定其ITS序列.结果 标记它们的ITS1,5.8 s,ITS2的全长序列,构建了葛根的ITS序列指纹图谱,通过分析比较,发现葛根属中野葛之间,野葛和粉葛之间有显著差异;粉葛之间的差异较小.野葛序列总的同一性为80.74%,长度差异可达20 bp,但可分为2个同一性为99.7%以上的组,提示来源可能为2个种.野葛和粉葛序列总的同一性为62%,最大长度差异可达180 bp.粉葛之间序列的同一性为98%以上,长度差异仅有4 bp.结论 以ITS序列测定与分析作为葛根超微饮片品种鉴定和质量控制标准是...

Objective To establish the identification and evaluation of ultramicro Radix Puerariae. Methods Radix Puerariae from 4 kinds of Pueraia （Willd.） Ohwi and 10 kinds of P. thomsonii Benth. which came from different regions were used as materials, their total DNA were extracted and cloned, and the ITS sequence was determined by molecular biological technique. Results Some remarkable differences among the group of Pueraia lobata （Willd.） Ohwi, and between the 2 groups of Pueraia lobata （Willd.） Ohwi and P. thomsonii Benth were found. But the d...