Objective To determine the anti-aging effect of hemp seed oil, protein and lignanamide. Methods We used the natural aged mice, fed them with difference doses of hemp seed oil (2. 5, 5, and 10 mL·kg^-1·d^-1), protein (1.3, 2.6g·kg^-1·d^-1) andlignanamides (0.11, 0.22 g·kg^-1·d^-1) every day for 30 d, and detected the total cholesterol (TC), triglyeride (TG), malonaldehyde (MDA) content and superoxide dismutase (SOD) activity in the blood serum of the old mice; the MDA content, SOD, glutathione peroxidase (GSH-Px) activity i...