Objective To screen endophytic fungi producing structural analogues evodiamine.Methods Plate method was used to separate and purify endophytic fungi in Euodia rutaecarpa from different origins like Xinhuang,Xiangxiang,Loudi of Hunan,Tongren Guizhou,Wanzhai Jiangxi and Wenzhou Zhejiang,respectively.TLC and LC-MS analysis were used to analyze the components such as alkali and rutaecarpine and structural analogues.Results A total of 31 endophytic fungi in Euodia rutaecarpa were separated from 6 Euodia rutaecarpa regions.Different types of endophytic fungi were separated in leaves,stems,and fruits...