期刊论文

Extraction and preparation of β-cyclodextrin inclusion compound of volatile oil from Radix Angelica Sinensis and Fructus Aurantii

中文

中南药学

1672-2981

2012

10

6

401-405

10.3969/j.issn.1672-2981.2012.06.001

湖南省科技厅项目（No.2009TP4051-1）； 长沙市科技计划项目（No.k1003034-31）； 湖南省高校“中药新药创制与资源综合持续利用”科技创新团队资助（No.湘教通[2010]212号）； 国家中医药管理局“药用植物学”、“中药药剂学”重点学科资助（No.国中医药发[2009]30号）； 湖南省“中药学”重点学科资助（No.湘教通[2011]76号）；

本校为第一机构

目的优选当归和枳壳挥发油的提取工艺及β-环糊精包合工艺。方法采用单因素考察法,以药材粉碎度、蒸馏时间、浸泡时间、加水量为考察因素,挥发油提取量为指标,优选其提取工艺。以挥发油包合率为指标,对饱和水溶液法、研磨法及超声法进行比较,采用正交实验法,优选出最佳包合工艺;并对包合物进行TLC及IR检验。结果最佳提取工艺为药材粉碎成粗粉（20目）,8倍量水,浸泡0.5h,提取5h;最佳包合方法是研磨法,挥发油和β-环糊精的比例为1∶8（mL∶g）,加4倍量的水,研磨3h。结论本法确定的最佳提取与包合工艺,可有效地提取与保留当归和枳壳的活性成分挥发油,为其进一步研究打下基础。

Objective To optimize volatile oil extraction and β-cyclodextrin inclusion compound from Radix Angelica Sinensis and FructusAurantii. Methods The single factor test was carried out to determine the volume of volatile oil by 4 factors： medicinal material crushing degree, distilling time, soaking time, and water quantity in Radix Angelica Sinensis and Fructus Au rantii. The ratio of oil in the inclusion complex was used to evaluate the technology based on orthogonal design. Rate of vola tile oil served as the evaluating indicator. Best inclusio...