期刊论文

中文

中南药学

1672-2981

2015

13

1

71-74

10.7539/j.issn.1672-2981.2015.01.020

(No.81203007):国家自然科学基金 (No.13JJ9010):湖南省自然科学基金 (No.14K071):湖南省教育厅科技创新平台

本校为第一机构

目的 建立高效液相色谱法对不同加工方法山银花药材中绿原酸、木犀草苷2个指标性成分进行含量测定,确定山银花最佳加工方法.方法 采用高效液相色谱法,色谱柱:AgiLent C18(4.6 mm×250 mm,5μm);流动相:乙腈-0.4％冰醋酸,梯度洗脱;流速:1 mL·min-1;检测波长:350 nm;柱温:25℃.结果 不同加工方法山银花中绿原酸、木犀草苷含量有明显变化,其中低温烘干与杀青烘干山银花中绿原酸、木犀草苷含量较高.结论 低温烘干法加工山银花外观质量最优,绿原酸、木犀草苷含量最高,应广泛推广应用.

Objective To establish an HPLC method to determine the content of chlorogenic acid and galuteolin in Flos Lonicerae by different drying ways, and to determine the best processing method for Flos Lonicerae. Methods The separation was performed on the Agilent C18（4.6 mm×250 mm, 5 μm） column with acetonitrile-0.4% glacial acetic acid as the mobile phase. The flow rate was 1 m L·min- 1. The wavelength of UV detector was 350 nm and the column temperature was 25 ℃. Results Chlorogenic acid and galuteolin content significantly changed in Flos Lo...