期刊论文

Quality standard of Qingwei Xiaoke capsules

中文

中南药学

1672-2981

2015

13

5

525-527

10.7539/j.issn.1672-2981.2015.05.021

本校为第一机构

目的 提高清胃消渴胶囊的质量标准.方法 采用Waters XTerra C18色谱柱(4.6 mm× 250 mm,5μm),流动相条件为乙腈和0.03％磷酸溶液梯度洗脱系统,流速为1.0 mL·min-1,检测波长为210nm,同时测定君药玄参中哈巴苷和哈巴俄苷的含量.结果 哈巴苷的线性范围在0.1920～1.728 μg,r=0.9990,平均加样回收率为98.4％,RSD为1.2％;哈巴俄苷的线性范围在0.060 48～0.5443 μg,r=0.9993,平均加样回收率为99.4％,RSD为2.0％.结论 所建立的方法具有专属、简便、准确、可重复的特点,增强了清胃消渴胶囊的质量可控性,提高、完善了原质量标准.

Objective To improve the quality standard of Qingwei Xiaoke capsules. Methods HPLC Waters XTerra C~8 column was used. The mobile phase was acetonitrile-0.03% phosphoric acid solution with gradient elution. The flow rate was 1.0 mL min 1. The detective wavelength was 210 rim. Harpagide and harpagoside of Figwort Root monarch drug in a prescription were simultaneously determined. Results The range of harpagide was 0.1920 -- 1.728 μg （r =0.9990）, the average recovery was 98.4% with RSD 1.2%, the range of harpagoside was 0.060 48 - 0.5443 gg （r...