期刊论文

Simultaneous determination of 5 components in Luohua Zizhu dispersible tablets by RP-HPLC

中文

中南药学

1672-2981

2016

14

5

532-535

10.7539/j.issn.1672-2981.2016.05.018

2015年湖南省教育厅科研项目（No.15C1378）；

本校为其他机构

目的 建立RP-HPLC法同时测定裸花紫珠分散片中5种主要有效成分的含量.方法 C18色谱柱(Gemini-NX,250 mm×4.6 mm,5μm),以0.1％磷酸(A)-乙腈(B)为流动相进行梯度洗脱,流速1.0 mL·min-1,检测波长340 nm.结果 建立了同时测定裸花紫珠分散片的主要有效成分木犀草素、木犀草苷、连翘酯苷B、毛蕊花糖苷和异毛蕊花糖苷的含量测定方法,阴性样品无干扰,样品分离好.结论 所建立的定量方法简便快速,准确度高,适合裸花紫珠分散片多指标组分的定量控制.

Objective To establish an RP-HPLC method to determine the content of 5 components in Luohua Zizhu dispersible tablets simultaneously. Methods The method was performed on a C18 chromatographic col- umn （Gemini NX, 250 mm × 4.6 mm, 5 pro）, with 0.1% phosphate acid （A）-acetonitrile （B） as the mobile phase in gradient elution, the flow rate was 1.0 mL·min - 1 and the detect wavelength was 340 nm. Results Five ingredients in Luohua Zizhu dispersible tablets was determined, including luoteolin, luteoloside, forsythoside B, acteoside and isoac...