Objective To analyze the character of 20 batches of Aurantii Fructus from different habitats. Methods We established the fingerprint of Hunan Aurantii Fructus and others Aurantii Fructus by HPLC, And analyzed the difference in different batches ofAurantii Fructus. Results Based on the area of common peak value, content difference was found. Conclusion Fingerprint analysis is accurate and reliable for Aurantii Fructus, which provides the basis for the identification of medicinal materi...