期刊论文

Content determination of sulfur dioxide residue in trichosanthin from different areas by optimization colorimetric method

中文

中南药学

1672-2981

2017

15

4

505-510

10.7539/j.issn.1672-2981.2017.04.028

公益性中医药行业科研专项（No.201407003）； 长沙市科技计划重点项目（No.k1406030-31）； 湖南省重点学科药学学科资助；

本校为第一机构

目的 通过优化比色法反应条件,建立比色分光光度法测定天花粉中二氧化硫的残留量.运用试剂盒法、比色法、顶空气相色谱法验证,探讨这3种方法测定结果的相关性.方法 采用紫外分光光度法测定试验最佳吸收波长;利用响应面分析法对影响含量的因素进行优化,在单因素试验的基础上选取试验因素与水平,根据中心复合设计试验原理,采用四因素三水平的响应面分析法确定最佳比色法反应条件.结果 确定最佳吸收波长为560 nm;最佳比色法反应条件:氨基磺酸铵溶液、氢氧化钠溶液、盐酸副玫瑰苯胺溶液用量分别为0.5、1.0、1.0 mL;反应时间为15 min.在该反应条件下,二氧化硫残留量在1.0～50.0 μg与峰面积线性关系良...

Objective To optimize the condition of colorimetric reaction and establish a spectrophotometric colorimetric method for the detection of sulfur dioxide residues in Trichosanthis Radix and to determine the correlation of these three methods, namely the reagent kit method, the colorimetric method, and the head space gas chromatography. Methods The optimal absorbing wavelength was determined by UV spectrophotometric. Response surface methodology was utilized to optimize factors which influenced the content of sulfur dioxide residues. According to ...