期刊论文

中文

中南药学

1672-2981

2019

17

3

352-355

10.7539/j.issn.1672-2981.2019.03.006

No.81573691:国家自然科学基金 No.81874507:国家自然科学基金 No.201612:湖南省中医药科研重点项目 No.16A162:湖南省教育厅科学研究重点项目 No.2018JJ2297:湖南省自然科学基金 No.2016JJ4065:湖南省自然科学基金 2011:湖南省重点学科建设项目 No.CX2018B509:湖南省研究生科研创新项目

本校为第一机构

目的免疫指纹图谱法研究双黄连注射剂中绿原酸的致敏性,并建立高效、快速筛查中药注射剂过敏原的检测方法。方法通过人工合成绿原酸完全抗原并用紫外光谱对其鉴定,所得抗原免疫动物制备抗体血清并对其分离纯化,用酶联免疫法检测绿原酸是否呈阳性,再用免疫指纹图谱法研究双黄连注射剂中绿原酸的免疫原性。结果紫外光谱的结果显示绿原酸抗原合成成功;酶联免疫法的结果显示绿原酸组颜色明显较阴性组深而呈阳性反应;运用免疫指纹图谱法检测绿原酸在双黄连注射剂中的致敏性,发现用IgE、IgG吸附前后绿原酸指纹图谱特征峰发生显著性变化,其吸附率分别为44.55%、38.42%。结论用酶联免疫法初步锁定绿原酸...

Objective To determine the sensitivity of chlorogenic acid in Shuanghuanglian injection by immune fingerprinting, and to establish an effective and rapid method to detect allergen in traditional Chinese medicine injection. Methods Complete antigen of chlorogenic acid was synthesized and identified by ultraviolet spectra. The antigens were immunized with animals to prepare antibody serum and purified. Enzyme-linked immunoassay was used to detect whether chlorogenic acid was positive or not. The immunogenicity of chlorogenic acid in Shuanghuanglian injection was studied by immun fingerprinting. ...