期刊论文

中文

中南药学

1672-2981

2019

17

6

825-830

10.7539/j.issn.1672-2981.2019.06.006

湖南省教育厅优秀青年项目(项目编号:16B033) 2014年湖南省校企合作人才培养示范基地项目(项目编号:湘教通[2014]272号) 新型药物制剂研发湖南省重点实验室培育基地(平台编号:2016TP1029)。

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目的 采用超高效液相色谱-四级杆-飞行时间串联质谱法(UPLC-Q-TOF-MS)对西洋参的主要化学成分及入血成分进行分析鉴定.方法 采用超高效液相色谱梯度洗脱分离各主要成分;电喷雾电离源负离子模式检测,四级杆飞行时间串联质谱法对各主要色谱峰进行归属.灌胃给予大鼠3 g·kg-西洋参粉,收集血清样品.采用UPLC-Q-TOF-MS分析并鉴定西洋参粉提取物及入血成分.结果 通过质谱信息并结合对照品数据及相关文献,提取物中共鉴定了22个化合物,大鼠含药血清中共鉴定了8个入血成分.结论 UPLC-Q-TOF-MS可较全面地对西洋参成分进行定性分析,为进一步阐明药效物质基础和建立全面的质量控制提供参考依据.

Objective To analyze and identify the main chemical components and serum components of American ginseng by UPLC-Q-TOF-MS. Methods The main components in the extracts were separated with the gradient elution method with UPLC. The negative electro spray ionization (ESI) source was used for the detection, the main chromatographic peaks were assigned by Q-TOF. Rats were intragastrically administered of American ginseng powder at 3 g·kg^-1 . The serum samples were collected and pretreated. UPLC-Q-TOF-MS was used to analyze and identify the extract ...