期刊论文

中文

中南药学

1672-2981

2024

22

03

660-667

10.7539/j.issn.1672-2981.2024.03.017

No.81974532:国家自然科学基金 No.A2023032:湖南省中医药管理局重点课题 No.CPA-Z05-ZC-2021-003:中国药学会医院药学专委会人才专项资助项目 No.GXZYC20230219:广西壮族自治区中医药管理局自筹经费科研课题

本校为第一机构

目的 探索雷公藤甲素（TP）双向调控Nrf2/p65产生毒性与保护的双重作用及机制。方法通过小分子与蛋白质对接模拟，分析TP与Nrf2/p65的潜在作用关系。在细胞实验里，采用不同浓度的TP分别干预人正常肝脏细胞（L02）与小鼠肝库普弗细胞（KC），采用MTT法检测细胞存活率，Western blot检测Nrf2、HO1、p65、IL-6的蛋白表达；在动物实验里，采用TP干预糖尿病肾病（DN）模型大鼠，HE染色法检测大鼠肾脏病理，靛酚蓝比色法检测血清BUN,ELISA法检测血清炎症因子TNF-α、IL-6与IL-1β的含量变化，RT-q PCR检测肾组织中p65、podocin和Kim-1的m RNA水平，Western blot检测肾组织中p65的蛋白表达，免疫组化法...

Objective To determine the dual role of triptolide(TP),in regulating both the toxicity and protection through the Nrf2/P65 pathway and related mechanism.Methods Molecular docking simulations were performed to analyze the potential interaction between TP and Nrf2/P65.In the cell experiments,different concentrations of TP were used to intervene human normal liver cells(L02)and mouse Kupffer cells(KC).The cell viability was assessed with the MTT assay,and the protein expression of Nrf2,HO1,P65,and IL-6 was examined by Western blot.In the animal ex...